Kits

DNA sequence information is being compiled by various genome initiatives and numerous research groups around the world. The management of this data is known as bioinformatics. This information is stored in various DNA sequence databases which can be readily accessed via the internet. In this experiment, students read x-rays containing DNA sequences which represent segments of important cellular genes. Using bioinformatics databases, students compare and extrapolate database information and identify the gene product. Kit includes: instructions, 3 sets of 4 autoradiograms. All you need: white light visualization system, computer access to the internet. For 12 Groups. Complete in 45 minutes.

Your students use primers for 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype! They can then compare their class results with others around the world over the internet. For 25 Students. Time required: Set up 30 min. PCR 2 hours or overnight Electrophoresis 45 min. Kit includes: instructions, proteinase K, PCR beads, control DNA and primers, microtubes, chelating agent, agarose, DNA ladder, practice gel loading solution, gel loading dye, electrophoresis buffer, gel stain. All you need: micropipettes to measure between and 50 µl, tips, waterbath, thermal cycler, electrophoresis tank and power supply.

In this experiment, students learn how to apply the gas laws to the function of the microrespirometer. Students will observe cell respiration of germinating seeds and describe the effects of temperature on the rate of cell respiration. Kit includes: instructions, 1 mL pipet, glass beads, peas, potassium hydroxide solution, cork stoppers, absorbent cotton, nonabsorbent cotton, plastic vials, parafilm. All you need: thermometers, trays (at least 14"" long), silicon glue, cork borer, tape, timers For: 10 groups.

Refill for FO91850 Blood Typing Kit.

When normal cells are grown in culture they stop growing when they become overcrowded. This is called contact inhibition. Cancer cells in culture grow in an uncontrolled way because they have lost this property. This helps tumors to form in the body. In addition, many different cell types can be present in single tumor. This experiment allows students to see the differences between normal and cancer cells in both their growth and cell types. Kit includes: instructions, multispot slides (2 cell types each), fixing agent, eosin and FlashBlue™ stain, mounting medium, cover slips, transfer pipets, immersion troughs. All You Need: Microscope for 400x magnification. For 6 Lab Groups. Complete in 35 minutes.

Cell Culture is vital technology used in life science research and in biotechnology laboratories. The study of basic cell biology, diseases and cancer, the development and testing of new therapeutics, and the production of new drugs relies on using the techniques introduced in this experiment. Students will learn how to grow eukaryotic cells in culture, basic cell staining and how to count cells. The techniques used in these experiments will provide the student with skill set desired in both academic research and industry. Kit Includes: instructions, growth media, flasks, Giemsa stain, trypan blue dye, sterile T25 flasks, sterile culture dishes, sterile large pipets, small pipets, cell counting chambers, Sf9 insect cells. All you need: microscope and spray bottle with 70% ethanol or methanol. For 6 Lab Groups. Basic cell culture techniques 30 min., Examination of insect cell cultures 30 min., Maintenance of insect cell cultures 1 hour, Cell Viability using trypan blue 30 min., Differential staining using giemsa stain overnight.

In this lesson, students explore how DNA technology can be used to explore the relationship between genotype and phenotype using one of two exciting scenarios (medical diagnostics or alien genetics). Fluorescent dyes simulate DNA fragments, eliminating post-electrophoresis staining and saving you valuable classroom time. Kit includes: instructions, Ready-to-Load™ dye samples, agarose powder, practice gel loading solution, electrophoresis buffer, microtipped transfer pipets. All you need: electrophoresis apparatus, power supply, microwave or hot plate, and black light (BI130920 recommended). For: 10 Groups

Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students will test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction. Kit includes: instructions, control DNA and primers, DNA ladder, proteinase K, PCR EdvoBeads, gel loading dye, agarose, electrophoresis buffer, InstaStain® Ethidium Bromide and FlashBlue™ stain. All you need: Automatic micropipets (5 - 50 µl) and tips, waterbath, ice, balance, microcentrifuge, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator. For: 25 Students

Caenorhabditis elegans is soil nematode that has great potential for educational research, partly because of its rapid (3-day) life cylce, small size (1.0 mm long adult), and ease of laboratory growth cultivation. Thousands of animals can grow on single petri dish seeded with lawn of Escherichia coli as the food source. Students will engage in an environmental toxicity scenario and will use pre-diluted concentrations of heavy metal solution to determine the effect on the worms. Kit includes: instructions, C. elegans-normal, C. elegans-Toxicity mutant, petri dishes, NGM medium, E. coli 0P50 Bactobeads™, cell counting chambers, buffer, pipets, sterile loops, tubes and heavy metal compounds. All you need: ethanol, timers, microscopes, covered box. For: 10 groups.

Give your students the opportunity to carry out PCR in the classroom! This kit provides easy to follow instructions for your students to develop various crime scene scenarios independently. Plasmid DNA, when amplified by PCR, provides products that represent individual DNA profiles. Your students can then solve a crime! Kit includes: instructions, PCR EdvoBeads, DNA templates, primers, DNA ladder, ultrapure water, wax beads, agarose, loading dye, electrophoresis buffer, InstaStain® Blue, and FlashBlue™ stain. All you need: micropipets to measure between 5 and 50 µL, tips, thermal cycler, electrophoresis apparatus, power supply, microwave or hot plate, UV transilluminator. For: 25 Students working in 5 groups

When DNA is subcloned in the pUC polylinker region, ß-galactosidase production is interrupted, resulting in the inability of cells to hydrolyze X-Gal. This results in the production of white colonies amongst background of blue colonies. This experiment provides DNA fragment together with linear plasmid and T4 DNA Ligase. Following the ligation to synthesize the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic resistant white and blue colonies are counted. ß-galactosidase activity is assayed from blue and white bacterial cells. This experiment can be broken down into three modules: ligation, transformation, and assay of ß-galactosidase. Kit includes: instructions, Linearized pUC plasmid DNA fragment, T4 Ligase, bacterial LyphoCells™ for transformation, reconstitution buffer, XGal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour™ Luria Broth Agar, Luria broth media for recovery, growth media, assay components, plastic supplies All you need: incubation oven, two waterbaths, shaking incubator or shaking waterbath, microwave or hot plate, automatic micropipet and tips, spectrophotometer, balance, centrifuge, microcentrifuge, glassware and cuvettes, distilled water, ice. For: 5 groups.

The Green Fluorescent Protein from the jellyfish Aequorea victoria is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment your students will transform bacteria either with GFP, BFP or both! Kit includes: instructions, cells, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour agar, Luria broth, petri dishes, sterile pipettes, BactoBeads™ and loops. All You Need: Waterbath, 37˚C incubation oven, microwave or hot plate, long wave UV lamp. For 10 Lab Groups, complete in 50 minutes and grow overnight.

Learn how immunoelectrophoresis identifies proteins based on their combined electrophoretic and immunological properties. This method is useful to monitor antigen and antigen-antibody purity and to identify a single antigen in a mixture of antigens. In this experiment, serum proteins are separated by agarose gel electrophoresis and the point of equivalence is observed by the antigenantibody complex formation. Kit includes: instructions, proteins, antibodies; reagents, agarose, buffer, transfer pipettes, well cutters, paper wicks. All you need: horizontal electrophoresis apparatus, power supply, automatic micropipettes with tips, waterbath, microwave or hot plate, incubation oven, laboratory glassware, microscope slides, paper towels, distilled water. For 10 Groups

Gene of Fortune™ Game. This novel ''Bingo'' game is an excellent resource to introduce concepts of the genetic code. The games can be played over several class periods. Concepts reinforced include the genetic code, single and three letter amino acid abbreviations, and the characteristics of amino acids. The game includes Gene of Fortune™ Spinner, 10 different cards, game chips, and instruction manual. Genetic Dice™ Game Using Genetic Dice™, students will have fun while they learn about DNA. This resource utilizes set of game boards, genetic dice, and game chips to reinforce concepts centering on Watson-Crick DNA base pair rules.

This kit allows students to extract their own DNA which can be placed in the tubes supplied and used as pendant on a necklace. Kit includes sufficient materials for 25 students and Teacher.

The mitochondria are thought to have evolved from symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, your students will amplify two regions of their mitochondrial DNA. For 25 Students Time required: Set up 30 min. PCR hours or overnight Electrophoresis 45 min. Kit includes: instructions, proteinase K, PCR beads, control DNA and primers, microtubes, chelating agent, agarose, DNA ladder, practice gel loading solution, gel loading dye, electrophoresis buffer, gel stain. All you need: micropipettes to measure between and 50 µl, tips, waterbath, thermal cycler, electrophoresis tank and power supply.

A real time saver, this experiment uses PCR to amplify small section of Lambda DNA via two-step process, saving valuable classroom time and allowing completion of the lab in one session. Kit includes: instructions, PCR beads, DNA template primers, DNA size ladder, ultrapure water, wax beads, gel loading dye, agarose, electrophoresis buffer, gel stain. All you need: 5-50 µL adjustable micropipettes, tips, thermal cycler, electrophoresis tank and power supply. For 10 Lab Groups Time required: Complete in 90 minutes.

Some foods contain raw materials from genetically modified organisms (GMO). Examples include tofu, corn flakes and corn meal. In this experiment, your students will extract DNA from food or plant material and perform PCR to determine if any GM indicator genes are present. Amplified DNA is separated and sized by agarose gel electrophoresis. Kit includes: instructions, DNA extraction reagents, PCR EdvoBeads™, microtubes, primers, DNA ladder, ultrapure water, wax beads, gel loading dye, agarose, electrophoresis buffer, InstaStain Ethidum Bromide and FlashBlue™ stain. Requires isopropanol. For 10 lab groups.

Using prestained LyphoProteins™, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained Proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers. Kit includes: instructions, denatured LyphoProtein™ samples, standard protein markers, gel loading solution, buffer, Protein InstaStain®. All you need: 3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, hot plate or burner, white light visualization system, automatic micropipet with fine tips, microtest tube holder, lab glassware, methanol, glacial acetic acid, distilled or deionized water. For 6 Groups sharing 3 Gels. Electrophoresis 60 min., Staining 20 min., Destaining 2 hours.

In this experiment, students actually purify the restriction enzyme, Eco RI! This procedure utilizes an ion exchange chromatography step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for college level courses. Kit includes: instructions, ion exchange matrix, chromatography columns, E.coli cell extract, equilibration elution buffer, Lambda DNA, Lambda/Eco RI Marker, KCl, glycerol, dilution reaction buffers, gel loading solution, agarose, electrophoresis buffer, stain. For 10 Lab Groups. Complete in 45 minutes. All you need: horizontal gel electrophoresis apparatus, power supply, UV visualization system, waterbath, microcentrifuge, microwave or hot plate, UV spectrophotometer cuvettes, automatic micropipet with tips, ring stands clamps, 10 mL pipettes, lab glassware, ice and ice buckets. For 5 Purifications Packing column 45 min., Restriction Analysis - 35 min., Restriction Analysis - 50 min., Gel Prep 30 min., Electrophoresis 30 min., Staining Destaining 2 min.

Introduce your students to the exciting science of DNA Sequencing. This kit contains the four Ready-to-Load sequenced DNAs (nucleotides A, C, G, & T) in an easy to use, safe format. Students load the four separate reactions into agarose gels, run the gels, stain them, and actually read the DNA sequence. This experiment can be used to introduce genome concepts and help your students gain better understanding of the science behind DNA sequencing. Kit Includes: instructions, Ready-to-Load™ QuickStrips™ DNA samples, UltraSpec-Agarose™ powder, practice gel loading solution, electrophoresis buffer, InstaStain® Blue and FlashBlue™ stain, calibrated pipette, and microtipped transfer pipettes. All you need: electrophoresis apparatus, power supply, automatic micropipette and tips, balance, microwave or hot plate, white light visualisation system. For 8 Gels. Complete in 55 minutes.