Purification Of The Restriction Enzyme Eco Ri. In this experiment, students actually purify the restriction enzyme, Eco RI! This procedure utilizes an ion exchange chromatography step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for college level courses.Kit includes: instructions, ion exchange matrix, chromatography columns, E.coli cell extract, equilibration elution buffer, Lambda DNA, Lambda/Eco RI Marker, KCl, glycerol, dilution reaction buffers, gel loading solution, agarose, electrophoresis buffer, stain.For 10 Lab Groups. Complete in 45 minutes.All You Need: horizontal gel electrophoresis apparatus, power supply, UV visualization system, waterbath, microcentrifuge, microwave or hot plate, UV spectrophotometer cuvettes, automatic micropipet with tips, ring stands clamps, 10 ml pipets, lab glassware, ice and ice buckets.For Purifications Packing column 45 min., Restriction Analysis - 35 min., Restriction Analysis - 50 min., Gel Prep 30 min., Electrophoresis 30 min., Staining Destaining 2 min.
