Chromatography Kits

Introduce chromatographic separation to your class and show them how dyes of different colours separate on the basis of their size and shape. Columns may be rinsed and reused. Kit includes: instructions, sample mixture, chromatography columns, dry matrix, elution buffer, transfer pipettes, microtest tubes. All you need: 50 or 100 mL beakers, 25m mL beaker or test tube, ring stands with clamps, distilled water. For 10 Separations. Packing Column 20 min., Column Separation 40 min.

This experiment introduces chromatographic theory and methods of thin layer chromatography. A mixture of dyes are separated on cellulose-based TLC plate using two different solvent systems. Kit includes: instructions, samples, reagents and solvents, 10x20 cm cellulose thin layer plate (to be cut into eight 5x5 cm squares), 5 µL glass capillary pipets. All you need: 250 mL beakers, metric rulers, pipet pump, or 10 mL pipets, distilled water. For 8 Separations. Spotting Plates 20 min., TLC Separation 5 min.

Most molecules have net charge within pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, mixture of two chemicals is absorbed onto solid support ion-exchange column and separated during elution under conditions that influence their net charge. Kit includes: instructions, ion exchanger, chemical mixture, potassium acetate buffer, chromatography columns. • All you need: spectrophotometer cuvettes, ring stands and clamps, test tubes, lab glassware, distilled water, ml pipets and pumps. For 6 Separations. Requires 60-90 min.