Transformation With Blue & Green Flourescent Proteins--Fridge&Freezer. The Green Fluorescent Protein from the jellyfish Aequorea victoria is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment your students will transform bacteria either with GFP, BFP or both!Kit includes: instructions, cells, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour agar, Luria broth, petri dishes, sterile pipettes, BactoBeads™ and loops. All You Need: Waterbath, 37˚C incubation oven, microwave or hot plate, long wave UV lamp.For 10 Lab Groups, complete in 50 minutes and grow overnight.
