Mitochondrial Dna Analysis Using Pcr--Frezzer. The mitochondria are thought to have evolved from symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, your students will amplify two regions of their mitochondrial DNA. For 25 Students Time required: Set up 30 min. PCR hours or overnight Electrophoresis 45 min. Kit includes: instructions, proteinase K, PCR beads, control DNA and primers, microtubes, chelating agent, agarose, DNA ladder, practice gel loading solution, gel loading dye, electrophoresis buffer, gel stain. All you need: micropipettes to measure between and 50 µl, tips, waterbath, thermal cycler, electrophoresis tank and power supply.
