Pcr-Based Alu-Human Dna Typing--Freezer. Your students use primers for 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype! They can then compare their class results with others around the world over the internet. For 25 Students Time required: Set up 30 min. PCR hours or overnight Electrophoresis 45 min.Kit includes: instructions, proteinase K, PCR beads, control DNA and primers, microtubes, chelating agent, agarose, DNA ladder, practice gel loading solution, gel loading dye, electrophoresis buffer, gel stain. All you need: micropipettes to measure between and 50 µl, tips, waterbath, thermal cycler, electrophoresis tank and power supply.
