Biology

When DNA is subcloned in the pUC polylinker region, ß-galactosidase production is interrupted, resulting in the inability of cells to hydrolyze X-Gal. This results in the production of white colonies amongst background of blue colonies. This experiment provides DNA fragment together with linear plasmid and T4 DNA Ligase. Following the ligation to synthesize the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic resistant white and blue colonies are counted. ß-galactosidase activity is assayed from blue and white bacterial cells. This experiment can be broken down into three modules: ligation, transformation, and assay of ß-galactosidase. Kit includes: instructions, Linearized pUC plasmid DNA fragment, T4 Ligase, bacterial LyphoCells™ for transformation, reconstitution buffer, XGal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour™ Luria Broth Agar, Luria broth media for recovery, growth media, assay components, plastic supplies All you need: incubation oven, two waterbaths, shaking incubator or shaking waterbath, microwave or hot plate, automatic micropipet and tips, spectrophotometer, balance, centrifuge, microcentrifuge, glassware and cuvettes, distilled water, ice. For: 5 groups.

The Green Fluorescent Protein from the jellyfish Aequorea victoria is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment your students will transform bacteria either with GFP, BFP or both! Kit includes: instructions, cells, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour agar, Luria broth, petri dishes, sterile pipettes, BactoBeads™ and loops. All You Need: Waterbath, 37˚C incubation oven, microwave or hot plate, long wave UV lamp. For 10 Lab Groups, complete in 50 minutes and grow overnight.

Learn how immunoelectrophoresis identifies proteins based on their combined electrophoretic and immunological properties. This method is useful to monitor antigen and antigen-antibody purity and to identify a single antigen in a mixture of antigens. In this experiment, serum proteins are separated by agarose gel electrophoresis and the point of equivalence is observed by the antigenantibody complex formation. Kit includes: instructions, proteins, antibodies; reagents, agarose, buffer, transfer pipettes, well cutters, paper wicks. All you need: horizontal electrophoresis apparatus, power supply, automatic micropipettes with tips, waterbath, microwave or hot plate, incubation oven, laboratory glassware, microscope slides, paper towels, distilled water. For 10 Groups

A set of coloured beads that can be designated to represent the Watson-Crick DNA bases (A, T, G, C). The beads can be used in variety of ways to demonstrate concepts related to the structure and biology of DNA.

Gene of Fortune™ Game. This novel ''Bingo'' game is an excellent resource to introduce concepts of the genetic code. The games can be played over several class periods. Concepts reinforced include the genetic code, single and three letter amino acid abbreviations, and the characteristics of amino acids. The game includes Gene of Fortune™ Spinner, 10 different cards, game chips, and instruction manual. Genetic Dice™ Game Using Genetic Dice™, students will have fun while they learn about DNA. This resource utilizes set of game boards, genetic dice, and game chips to reinforce concepts centering on Watson-Crick DNA base pair rules.

This kit allows students to extract their own DNA which can be placed in the tubes supplied and used as pendant on a necklace. Kit includes sufficient materials for 25 students and Teacher.

The mitochondria are thought to have evolved from symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, your students will amplify two regions of their mitochondrial DNA. For 25 Students Time required: Set up 30 min. PCR hours or overnight Electrophoresis 45 min. Kit includes: instructions, proteinase K, PCR beads, control DNA and primers, microtubes, chelating agent, agarose, DNA ladder, practice gel loading solution, gel loading dye, electrophoresis buffer, gel stain. All you need: micropipettes to measure between and 50 µl, tips, waterbath, thermal cycler, electrophoresis tank and power supply.

A real time saver, this experiment uses PCR to amplify small section of Lambda DNA via two-step process, saving valuable classroom time and allowing completion of the lab in one session. Kit includes: instructions, PCR beads, DNA template primers, DNA size ladder, ultrapure water, wax beads, gel loading dye, agarose, electrophoresis buffer, gel stain. All you need: 5-50 µL adjustable micropipettes, tips, thermal cycler, electrophoresis tank and power supply. For 10 Lab Groups Time required: Complete in 90 minutes.

Some foods contain raw materials from genetically modified organisms (GMO). Examples include tofu, corn flakes and corn meal. In this experiment, your students will extract DNA from food or plant material and perform PCR to determine if any GM indicator genes are present. Amplified DNA is separated and sized by agarose gel electrophoresis. Kit includes: instructions, DNA extraction reagents, PCR EdvoBeads™, microtubes, primers, DNA ladder, ultrapure water, wax beads, gel loading dye, agarose, electrophoresis buffer, InstaStain Ethidum Bromide and FlashBlue™ stain. Requires isopropanol. For 10 lab groups.

Using prestained LyphoProteins™, subunit molecular weights are determined by analysis using denaturing SDS vertical polyacrylamide gel electrophoresis. Prestained Proteins with unknown molecular weights are assigned molecular weights based on the relative mobility of prestained standard protein markers. Kit includes: instructions, denatured LyphoProtein™ samples, standard protein markers, gel loading solution, buffer, Protein InstaStain®. All you need: 3 polyacrylamide gels (12%), vertical gel electrophoresis apparatus, power supply, hot plate or burner, white light visualization system, automatic micropipet with fine tips, microtest tube holder, lab glassware, methanol, glacial acetic acid, distilled or deionized water. For 6 Groups sharing 3 Gels. Electrophoresis 60 min., Staining 20 min., Destaining 2 hours.

In this experiment, students actually purify the restriction enzyme, Eco RI! This procedure utilizes an ion exchange chromatography step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for college level courses. Kit includes: instructions, ion exchange matrix, chromatography columns, E.coli cell extract, equilibration elution buffer, Lambda DNA, Lambda/Eco RI Marker, KCl, glycerol, dilution reaction buffers, gel loading solution, agarose, electrophoresis buffer, stain. For 10 Lab Groups. Complete in 45 minutes. All you need: horizontal gel electrophoresis apparatus, power supply, UV visualization system, waterbath, microcentrifuge, microwave or hot plate, UV spectrophotometer cuvettes, automatic micropipet with tips, ring stands clamps, 10 mL pipettes, lab glassware, ice and ice buckets. For 5 Purifications Packing column 45 min., Restriction Analysis - 35 min., Restriction Analysis - 50 min., Gel Prep 30 min., Electrophoresis 30 min., Staining Destaining 2 min.

Introduce your students to the exciting science of DNA Sequencing. This kit contains the four Ready-to-Load sequenced DNAs (nucleotides A, C, G, & T) in an easy to use, safe format. Students load the four separate reactions into agarose gels, run the gels, stain them, and actually read the DNA sequence. This experiment can be used to introduce genome concepts and help your students gain better understanding of the science behind DNA sequencing. Kit Includes: instructions, Ready-to-Load™ QuickStrips™ DNA samples, UltraSpec-Agarose™ powder, practice gel loading solution, electrophoresis buffer, InstaStain® Blue and FlashBlue™ stain, calibrated pipette, and microtipped transfer pipettes. All you need: electrophoresis apparatus, power supply, automatic micropipette and tips, balance, microwave or hot plate, white light visualisation system. For 8 Gels. Complete in 55 minutes.

An economical way to introduce DNA electrophoresis to the classroom. This LabStation™ provides all the equipment you need to run any of our DNA or dye electrophoresis kits with your students. Supports 24 students. Contents include: •1 x M36 Hexagel™ Electrophoresis Apparatus (six 7 x 7 cm trays) •1 x DuoSource™ 150 (75/150 V for 1 or 2 units) •2 x Fixed volume MiniPipette (40 µl) •1 x Yellow Micropipette tips (1 - 200 µl / 2 Racks of 96) •1 x DNA fingerprinting classroom kit

50-fold concentrated solution (Tris-acetate, EDTA, pH 7.8).

50-fold concentrated solution (Tris-acetate, EDTA, pH 7.8).

Microtest Tube rack which holds 80 1.5/2.0 mL tubes. Colour may vary.

PCR test tubes. 1.5 mL capacity.

Thin-walled PCR microtest tubes. The 0.2 mL tubes are suitable for use in the Edvocycler. Sold in packs of 100.

Tris-borate, EDTA buffer solution for electrophoresis. Sufficient to make 5 litres of working buffer.

Agarose powder for electrophorisis.

Agarose powder for electrophorisis.

Yields 6 mL final volume of DNA sample. Recommended usage: Add 1 volume solution to 9 volumes of sample and mix well.

A bottle of 25 beads. Each PCR EdvoBead™ contains: • Taq DNA Polymerase • Taq DNA Polymerase buffer, dNTP Mixture, MgCl2 Store at room temperature with desiccant.

Save time, money, the environment...and get better gel results! SYBR Safe® is a DNA stain that fluoresces with a bright green colour when excited with UV light. Like Ethidium Bromide, SYBR Safe® binds specifically to the DNA double helix. However, unlike Ethidium Bromide, SYBR Safe® has been engineered to be less mutagenic than Ethidium Bromide, making it much safer to use, particularly in the classroom. • SAFE for the biotechnology classroom • More sensitive than ethidium bromide • Non-mutagenic • 10,000 x concentrate for 750 mL • Volume: 80 µL

This Electrophoresis Reagent package contains enough reagents to perform up to 40 electrophoresis experiments with the EDVOTEK Electrophoresis Apparatus Model M6, M12 or M36 based upon an agarose gel concentration of 0.8%. The EDVOTEK proprietary formulation of FlashBlue™ staining solutions makes total of 1.2 litres of working stain. Enough gel loading solution is provided to prepare 150 samples (40 µL each) for electrophoresis. Kit includes: UltraSpec-Agarose™ (10 grams), 100 mL Electrophoresis Buffer (50 x), 1ml Gel Loading (10 x) Solution with tracking dye, FlashBlue™ stain (for 1.2 L)

Supports up to 4 students. Kit includes: • 1 x M12 Complete Electrophoresis Package • 1 x DuoSource™ 75 Power supply • 1 x Fixed Volume Mini Pipette (35 µl) • 1 x Yellow Micro Pipette Tips • 1 x Whose DNA Was Left Behind?

Kit includes: • 1 x EdvoCycler™ 2 (48 x 0.2 mL) • 6 x M12 Complete Electrophoresis Package (one 14 x 7 cm tray and two 7 x 7 cm trays) • 3 x QuadraSource™ Power Supply • 1 x TruBlu™ LED Transilluminator • 6 x Variable Micro Pipettes (5-50 µl) • 1 x 3 L Water bath • Supports up to 25 students

96-well plastic microtiter plates. Opaque white colour facilitates easy viewing of colour reactions. Kit includes: Set of 6 plates

Laboratory safety goggles with UV light protection.

This improved TruBlu™ 2 transilluminator features an enlarged viewing surface and a novel white light mode. Blue light enables visualization of SYBR® Safe stained DNA gels and white light enhances visualization of blue stained DNA or protein gels. It may also be used to brilliantly illuminate fluorescence in green fluorescent proteins (gfp). The TruBlu™ 2 has enough surface area to simultaneously view up to eight 7 x 7 cm gels and combines the functions of two units into one! •High density LED dual blue/white light modes •Large viewing surface: 270 x 150 mm •Orange contrast lid •Safe non-UV wavelength •Built-in fan minimises condensation •Blue light emission spectrum centred around 470 nm

This White LED Transilluminator features a spacious 250 x 250 mm viewing area illuminated by long life LEDs and is housed in a slim aluminium body. It is designed to safely enhance the visualization of DNA stained with FlashBlue™, proteins stained with Coomassie Blue and autoradiograms. Features: • 250 x 250 mm viewing area • 90 Lumens LED chips • Power Rating: 8 Watts • Universal voltage for worldwide use • 3 year warranty

This excellent value UV torch is durable and lightweight torch with super bright LEDs. Ideal for general UV use this device emits light at 385 nm frequency. This allows you to clearly detect invisible organics and inorganics in red, yellows, greens and blues. This torch will also verify currency, passports, driving licences etc. Great value for quality UV torch. Supplied with wrist strap and full information sheet.

This popular UV LED Torch has 41 LEDs with high quality and stable 380nm output and is suitable for multitude of different UV applications. As used by UK Police and Customs and Excise. Requires x AAA batteries, which should last for up to hours continuous operation.

Soft Wood. Dimensions: 550 x 400 mm with raised edges.

High-quality aluminium dissecting dishes with sloping sides. Dimensions: 300 x 200 x 50 mm.

High-quality aluminium dissecting dishes with sloping sides. Dimensions: 30 x 20 x 5 cm.With wax.

A useful cutting board for dissection and other applications, which is easy to clean, dishwasher safe and autoclavable. Product also has good chemical resistance and is virtually unbreakable and non-absorbent. • Autoclavable at 121 °C • Dishwasher safe • Good chemical resistance • Unbreakable in normal use • Non-absorbent and easy to clean • Dimensions: 300 x 210 x 9 mm

To hold 14 dissecting instruments with tie tapes.

Stainless steel.

A pack of 10 disposable blunt forceps, nylon.

Stainless steel. Straight with fine points and guide pin.

Stainless steel. With fine points and guide pin.

Spencer Wells stainless steel artery forceps. Straight with box joint, 225mm length.

Straight, stainless steel needle with plastic handle.

Straight, stainless steel needle with plastic handle.

Straight, stainless steel needle with stainless steel handle.

Aluminium with brass chuck.

Aluminium with brass chuck.

Stainless steel spike.

A pack of 100 setting pins.