Ion Exchange Chromatography. Most molecules have net charge within pH range of to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, mixture of two chemicals is absorbed onto solid support ion-exchange column and separated during elution under conditions that influence their net charge.Kit includes: instructions, ion exchanger, chemical mixture, potassium acetate buffer, chromatography columns.• All You Need: spectrophotometer cuvettes, ring stands and clamps, test tubes, lab glassware, distilled water, ml pipets and pumps. For Separations. Requires 60-90 min.
