Principles Of Thin Layer Chromatography. This experiment introduces chromatographic theory and methods of thin layer chromatography. mixture of dyes are separated on cellulose-based TLC plate using two different solvent systems. Kit includes: instructions, samples, reagents and solvents, 10x20 cm cellulose thin layer plate (to be cut into eight 5x5 cm squares), µL glass capillary pipets.All You Need: 250 mL beakers, metric rulers, pipet pump, or 10 mL pipets, distilled water. For Separations. Spotting Plates 20 min., TLC Separation 5 min.